Production (A) and release (B) of cytokines in murine astrocyte/neuron/microglia co-cultures. These co-cultures were pretreated with 210 nM C16 or its DMSO vehicle and exposed or not to 20 μM Aβ42 for 72 h in serum-free medium. TNFα, IL-1β and IL-6 were assessed by ELISA. Data are expressed as mean ± SEM of pg/mg protein for production and pg/mL for release (n = 5 in duplicate). *p < 0.05, **p < 0.01, ***p < 0.001 compared to DMSO; #p < 0.05, ##p < 0.01 and ###p < 0.001 compared to Aβ42 DMSO after one-way ANOVA with Newman-Keuls multiple comparison test.