Figure 8

C16 prevented apoptosis in neurons. Confocal staining of annexin V-FITC (green channel) with MAP2 (red channel) and GFAP (blue channel) (A, B, C and D) or with CD68 (red channel) (E, F, G and H) after 20 μM Aβ42 exposure and treated with 210 nM of C16 or DMSO for 72 h. A, B, E and F are merged images and C, D, G and H show only annexin-V-FITC staining. DAPI was used as a nuclear stain. A strong annexin V-FITC staining colocalizes with MAP2 staining after Aβ42 exposure, whereas GFAP-positive cells appear unaffected (A, C). We found also a diffuse and very weak staining of annexin V-FITC in CD68-positive cells (E, G). C16 prevented translocation of phosphatidylserine from the inner to the outer plasma membrane leaflet both in neurons (B, D) and microglia (F, H). Photos are representative of 4 experiments. Scale Bars: 40 μm.