EAE disease course in C57BL/6 mice after adoptive transfer of day 12 LNC. The successful protocol required LNC that were harvested from donor mice that had been immunized 12 days previously and had been treated with PT. Following LNC isolation, the cells were restimulated in vitro with MOGp35-55 (10 μg/ml) and IL-12 (0.5 ng/ml) for 72 hours. Error bars represent ± SD. Clinical scores shown are from at least two independent experiments. The difference between the successful experimental paradigm (●)and all unsuccessful paradigms (O) with regard to disease activity was significant, starting on day 7 (p ≤ 0.001).