Figure 4

Determining cytokine secretion by in vivo polarized T lymphocytes. LNC and SPC from donor mice immunized 6 or 12 days previously were stimulated directly ex-vivo with PMA (50 ng/ml) and ionomycin (750 ng/ml) to determine the frequency of recently activated CD3⁺CD4⁺CD44⁺ T cells present in these tissues prior to in vitro restimulation with antigen. IFNγ and Il-17 expressing T cells in LNC and SPC at day 6 and day 12 are shown in panels B, D, F, and H. Isotype controls are shown in panels A, C, E, and G. Data are representative of at least three independent experiments.