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Figure 7 | Journal of Neuroinflammation

Figure 7

From: Lymph node-derived donor encephalitogenic CD4+T cells in C57BL/6 mice adoptive transfer experimental autoimmune encephalomyelitis highly express GM-CSF and T-bet

Figure 7

Determining MOG-specificity in donor LNC and SPC. At day 6 or day 12, LNC and SPC were harvested and cultured for 72 hours in the presence of MOGp35-55 and IL-12 and prepared for tetramer staining. CD3+CD4+CD44+ T cells were examined for staining with MOG38-49 MHC class II tetramer, or control MHC class II tetramers containing hCLIP103-117. The percentage of MOG38-49 MHC class II tetramer positive cell is indicated in the regions B, D, F, & H. Control MHC class II tetramers containing hCLIP103-117 staining is shown in panels A, C, E, and G. Data shown are representative of at least 3 independent experiments.

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