Figure 2

MIF accumulates in neurons and astrocytes of the peri-infarct region during the first 7 days after tMCAo, in C57BL/6 mice. (A) Bright field micrographs. Representative image of MIF immunoreactivity (MIF-ir) in the peri-infarct motor/somatosensory cortex at 7 days after tMCAo. Scale bar: 100 μm (right). A higher magnification image denotes the expression in neurons and glia-like cells. Note the thick glia-like processes positive for MIF around the infarct core. Scale bar: 50 μm (left). (B) Confocal micrographs. Co-localization of MIF (Cy3, red) with the neuronal markers Tuj-1 and PV (both Cy5, green). Co-localization of MIF (Cy3, red) and the marker GFAP (Cy5, green) confirms the presence in reactive astrocytes. Scale bar: 50 μm. Infarct core-IC. (C) Confocal micrographs. Lack of co-localization of MIF (Cy3, red) and GST-π (Cy5, green) in the peri-infarct somatosensory-motor cortex at 7 days after occlusion. Scale bars: 50 μm and 20 μm (lower and higher magnification, respectively).