Figure 9

Number of CD74 ⁺ cells in WT and MIF-KO brains 7 days after tMCAo. (A and A1) Epifluorescence micrographs. ( A) Representative composite image of CD74 immunoreactivity in the mouse brain 7 days post-occlusion (WT). Note the presence of CD74⁺ cells essentially within the infarct core. (A1) Higher magnification micrographs denote the morphology of CD74⁺ cells. Scale bar: 10 μm. (B) Confocal images. Lack of co-localization MIF (Alexa 488, green)/CD74 (Cy3, red) in the infarct core. Scale bars: 50 μm and 10 μm (lower and higher magnification, respectively). (C) Number of CD74⁺ cells detected per ipsilateral brain hemisphere (sum of the analyzed brain levels) - WT and MIF-KO mice 7 days post-occlusion of the MCA (mean ± STD).