Figure 2

Spheroid myelinating cultures were prepared from fetal rat telencephalon and demyelinated with lysophosphotidyl choline on DIV 25-29 in the presence or absence of fingolimod. Confocal and electron microscopy was carried out to determine morphology of myelinated fibres. Spheroids were fixed and stained whole with specific antibodies against myelin basic protein (green) and neurofilament (red) after demyelination (A) and following remyelination (B). Spheroids prepared for electron microscopy were imaged to assess morphology prior to demyelination (C) and following remyelination (D).