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Figure 5 | Journal of Neuroinflammation

Figure 5

From: Fingolimod modulates microglial activation to augment markers of remyelination

Figure 5

Spheroid myelinating cultures were prepared from fetal rat telencephalon and demyelinated with lysophosphotidyl choline on DIV 25-29 in the presence or absence of fingolimod. Pro-caspase 3 and caspase 3 levels were assessed in homogenized spheroids with western blot (A, B) following homogenization and separation on polyacrylamide gel (n = 4; ** = p < 0.01; *** = p < 0.001). Caspase 3 and 7 activity were assessed in intact spheroids using the CaspaTag staining system (C, D) followed by fluorophore conjugation and confocal microscopy (n = 8; ** = p < 0.01). Results were analysed by ANOVA with Holm-Sidak post test.

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