MCP-1 induces microglia proliferation. Microglia were incubated for 24 h with fresh media (control), MCP-1 (10-200 ng/ml) or LPS 0.1 μg/ml. Cell proliferation was assayed using a BrdU kit as described in the Methods section. Data are expressed as percentage of control values (set to 100%); ***p < 0.0001 versus control, **p < 0.001 versus control, *p < 0.005 versus control. Data are means ± SE of n = 12 replicates per group.