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Figure 4 | Journal of Neuroinflammation

Figure 4

From: CCL2/MCP-1 modulation of microglial activation and proliferation

Figure 4

Expression of pro-inflammatory cytokines. (A) Microglia were incubated for 24 h with fresh media (control), LPS 1 μg/ml, MCP-1 50 ng/ml or a combination of LPS and MCP-1 at the indicated concentrations. RNA was isolated and MIP-1α, IL-1β and IL6 mRNA levels determined by RT-PCR. Data are expressed as percentage of control values (set to 100%); ***p < 0.0001 versus control. Data are expressed as mean ± SE for n = 6 replicates per group. (B) Microglia were incubated for 24 h with fresh media (control), LPS 1 μg/ml, MCP-1 50 ng/ml or a combination of LPS and MCP-1 at the indicated concentrations. IL-1β and IL-6 levels in the media were assessed by ELISA. ***p < 0.0001 versus control. Data are expressed as mean ± SE for n = 10 replicates per group.

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