Effect of GSNO and SIN-1 on levels of 3-NT and NO in plasma at 4 h after TBI. Levels of 3-NT (A) and NO (B) were measured in plasma using colorimetric and fluorometric assay kits, respectively. While GSNO treatment of TBI decreased, the SIN-1treatment increased the levels of 3-NT (A). GSNO treatment also increased the levels of NO, whereas SIN-1 treatment did not alter the levels of NO in the injured animals. Results are expressed as nM for 3-NT and μmol/L for NO. Data are presented as mean ± SD of triplicate determinations from five different experiments. ***p < 0.001 vs. Control, Sham, GSNO, +p < 0.05 vs. TBI.