Effect of GSNO on reduction of inflammation, demyelination and axonal loss at 14
th day after TBI. IHC photomicrographs of H&E staining (A) show enhanced inflammatory infiltration in the traumatic penumbra region of TBI group compared with GSNO group. Sham group does not show infiltration. LFB (B) and Bielschowsky silver (C) stainings show loss of myelin and axons respectively in the traumatic penumbra region. Treatment with GSNO reduced the loss of myelin and damage to axons. The sham brain does not show loss of myelin or axons. Photomicrographs are representative of n = 3 in each group.