Figure 3

The p38α MAPK inhibitor 069A attenuates the IL-1β and TNFα increase in response to diverse TLR ligands. The IL-1β (A) and TNFα (B) response from BV-2 cells was measured after 18 hrs of stimulation with either: (TLR2) 10 μg/ml LTA; (TLR3) 50 μg/ml poly(I:C); (TLR4) 100ng/ml LPS; (TLR7/8) 500ng/ml CL097; or (TLR9) 500ng/ml ODN1668. Treatment of cells with 4 μM of the p38α inhibitor, 069A, led to a significant reduction in the levels of IL-1β (C) and TNFα (D) in response to each of the different TLR ligands. The white bar represents the BV-2 cells stimulated with the ligand but without 069A (normalized to 100%). The black bar represents the BV-2 cells treated with TLR ligand + 069A. Asterisk denotes significance (* = p < 0.05, ** = p < 0.01, or *** = p < 0.001) for ligand-stimulated without 069A (white bar) compared to stimulated with 069A (black bar). Data represent two independent experiments.