Figure 6

Oral administration of 069A suppresses LPS-induced cortical IL-1β and serum TNFα in vivo : C57Bl/6 mice were administered either saline vehicle or 069A (5mg/kg) by oral gavage one hr prior to an intraperitoneal LPS (1mg/kg) injection. Serum and cortex were harvested at 90 min and 6 hrs, respectively, after LPS injection. (A) At the 6 hr timepoint, IL-1β levels were significantly lower in the cortex of mice treated with 069A compared to vehicle-treated mice. TNFα levels in the cortex were at or near the limit of detection of the assay. (B) At the 90 min timepoint, TNFα levels were significantly lower in the serum of mice treated with 069A compared to vehicle-treated mice. Serum IL-1β levels were low, and there was no inhibition by 069A at this timepoint. Asterisk denotes significance (* = p < 0.05) for vehicle-treated mice (white bar) compared to 069A-treated mice (black bar). N = 5-8 per group.