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Figure 2 | Journal of Neuroinflammation

Figure 2

From: Cooperative contributions of Interferon regulatory factor 1 (IRF1) and IRF8 to interferon-γ-mediated cytotoxic effects on oligodendroglial progenitor cells

Figure 2

Unlike IFNγ, IFNβ does not induce OPC apoptosis. A-B, Numbers of Annexin-V+/PI- (preapoptotic) cells and Annexin-V+/PI+ (dead) OPCs after treatment with IFNβ or IFNγ for 24h and 48 h. Annexin-V-/PI- (live), preapoptotic, and dead cells were counted by flow cytometry, and shown as percentages of averaged live cell numbers in control at 24 h. C, Caspase-3-like protease activity was measured with the fluorogenic substrate, Ac-DEVD-MCA, in the protein lysates of OPCs treated with GM alone (control, open circles), IFNβ (closed triangles) or IFNγ (closed circles) for 12, 24, and 48 h. ** indicates p < 0.01 compared with control or in comparison between the two groups indicated. D-E, Dose-dependent effects of IFNβ on induction of caspase-3-like proteinase activity and IGTP mRNA in OPCs. OPCs were treated with IFNβ at 0, 0.3, 1, and 3 kU/ml for 24 h, and activity of caspase-3-like proteinase (D) and IGTP mRNA (E) were quantified.

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