Figure 6
Expression constructs used in the study. A, PCMV-IE, immediate-early cytomegalovirus promoter; IRF1 DBD, IRF1 DNA binding domain; IRES, internal ribosome entry site; hrGFP, humanized Renilla GFP; pA, poly adenylation signal sequence. B, Fluorescence signals of hrGFP in the transfected cells became detectable by microscopy as early as 6 h after transfection. C, Fluorescent imaging demonstrates that the IRF1DN-hrGFP fusion protein was localized in the nuclei of transfected OPCs.