The effects of C5aR antagonist PMX53 on heat and mechanical nociception after incision. Intraplantar injection of PMX53 (30 μg/15 μl) reduced heat hyperalgesia and mechanical allodynia induced by hind paw incision (A and B, respectively). The first injection of PMX53 was administered before paw incision, the second and subsequent administrations were made 2 hours prior to each test session. For panels A and B values are expressed as mean ± SEM, *, P < 0.05 or ***, P < 0.001 compared to the control group. An arrow head at time = 0 indicates incision.