Incision alters levels of C5 and C5aR after hind paw incision. Samples for C5 and C5aR mRNA analyses were collected at the indicated time points. Data are displayed for peri-incisional skin (A and B), spinal cord (C and D) and DRG (E and F). For each tissue n = 4 samples, each analyzed in triplicate. Statistical comparisons were made between normalized baseline expression levels and those measured at the indicated time points. Data are presented as mean ± SEM. *, P < 0.05, ***, P < 0.001.