Effects of C5a on ongoing activities of mechanosensitive C-fibers in incised tissue. (A) Example recordings of C-fibers with receptive fields ≤ 2 mm from the incision before and after application of PBS-vehicle or C5a 1 μg/ml. Artifacts are produced during replacement of solutions as marked. (B-D) Ongoing activities of C-fibers before and after exposure to C5a (1 or 0.1 μg/ml) or PBS-vehicle which have receptive fields ≤ 2 mm (C) and > 2 mm (D) from the incision and in the sham group (B). Each line on the graphs represents a single unit. * indicates an increase of ongoing activity after drug application. The small horizontal lines in each graph indicate median values.