Effects of PBS-vehicle and C5a applied after PMX53 on ongoing activities of mechanosensitive C-fibers in incised tissue. (A) Example recordings of C-fibers with receptive fields ≤ 2 mm from the incision before and after application of PMX53 plus PBS-vehicle or PMX53 plus C5a 1 μg/ml. After recording of baseline, PMX53 1.8 μg/ml was applied, then PBS-vehicle or C5a was added to make the final concentration of PMX53 0.9 μg/ml. Artifacts are produced during replacement of solutions as marked. (C and E) Ongoing activities of C-fibers before and after exposure to PMX53 0.9 μg/ml plus PBS-vehicle or PMX53 0.9 μg/ml plus C5a 1 μg/ml which have receptive fields ≤ 2 mm (C) and > 2 mm (E) from the incision. For comparison, PBS-vehicle data from Figure 5C and D are in used in Figure 6B and D. Each line on the graphs represents a single unit. * indicates an increase in ongoing activity by drug application. Small horizontal lines in each graph indicate median values.