Effects of PMX53 pre-treatment on heat responses of mechanosensitive C-fibers in incised tissue. (A) Example recordings of C-fibers with receptive fields ≤ 2 mm from the incision after application of PMX53 0.9 μg/ml plus PBS-vehicle or PMX53 0.9 μg/ml plus C5a 1 μg/ml. (B-D and F-H) Heat responses of C-fibers after exposure to PMX53 0.9 μg/ml plus PBS-vehicle or PMX53 0.9 μg/ml plus C5a 1 μg/ml which have receptive fields ≤ 2 mm (B-D) and > 2 mm (F-H) from the incision. The proportion of C-fibers responsive to heat after application of each drug (E and I). Heat thresholds (B and F) and total action potentials for 20 s during heat application (C and G) and 40 s after heat application (D and H). PBS-vehicle data B-I are from Figure 7F-M. Each symbol represents a single unit. Data are presented as median values.