Figure 12
Seizure-induced alteration of cell-to-cell signalling/interaction, nervous system development/function, and cell morphology gene network. As detailed in Figure 5, a two-way ANOVA was performed to identify genes most significantly changed based on exposure and time after seizure onset. Genes meeting a p-value cutoff of 3.046 × 10-7 were overlaid onto a global molecular network developed from information within the IPA Knowledge Base, and the networks were then algorithmically generated based on their connectivity. (A) Graphical representation of the network reveals significant changes in gene expression due to sarin-induced seizure. Genes are represented as nodes of various shapes to represent the functional class of the gene product, and the biological relationship between two nodes is represented as a line. The intensity of the node color indicates the degree of differential expression. (B) Top three genes within network significantly altered by sarin-induced seizure. Moesin (MSN) and leucine rich repeat (in FLII) interacting protein 1 (LRRFIP1) expression increased over the time course with peak expression at 24 h after seizure onset, while brain derived neurotophic factor (BDNF) expression peaked at 3 h after seizure onset and dropped slightly at 6 and 24 h.