Figure 5

Seizure-induced alteration of cell-to-cell signaling and interaction, inflammatory response, and cellular movement gene network. A two-way ANOVA was performed to identify genes most significantly changed based on exposure (saline or sarin) and time after seizure onset interaction. Each probeset ID and corresponding false discovery rate (FDR) corrected p-value was imported into IPA and mapped to its corresponding gene in the IPA Knowledge Base. A p-value cutoff of 3.046 × 10^-7 was set to limit the number of molecules considered for the analysis and identify genes whose expression was significantly differentially regulated. Genes meeting the cutoff criteria were overlaid onto a global molecular network developed from information within the IPA Knowledge Base, and the networks were then algorithmically generated based on their connectivity. Graphical representation of the network reveals significant changes in gene expression due to sarin-induced seizure. Genes are represented as nodes of various shapes to represent the functional class of the gene product, and the biological relationship between two nodes is represented as a line. The intensity of the node color indicates the degree of differential expression.