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Figure 1 | Journal of Neuroinflammation

Figure 1

From: Transcriptional responses of the nerve agent-sensitive brain regions amygdala, hippocampus, piriform cortex, septum, and thalamus following exposure to the organophosphonate anticholinesterase sarin

Figure 1

Principal component analysis reveals sample partitioning based on brain region and time following seizure onset. Rat amygdala, hippocampus, piriform cortex, septum, and thalamus were collected at the specified times after seizure onset and processed for oligonucleotide microarray analysis. The raw signal intensities were normalized using the RMA algorithm and visualized using PCA to identify major sources of variability in the data. Each point on the PCA represents the gene expression profile of an individual animal. Point shape corresponds to exposure condition, point color corresponds to the time after seizure onset at which the tissue was collected, and point size indicates absence or occurrence of sarin-induced seizure. Ellipsoids highlight partitioning of samples based on brain region (A) and time point following seizure onset at which the tissues were collected (C). The principal components in the three-dimensional graph represent the variability in gene expression levels seen within the dataset. Principal component 1 (PC#1, x-axis) accounts for 17.10% of the variability in the data; PC#2 (y-axis) represents 12.00% of the variability; and PC#3 (z-axis) represents 9.35% of the variability in gene expression levels seen within the dataset.

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