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Figure 1 | Journal of Neuroinflammation

Figure 1

From: Myelin-phagocytosing macrophages modulate autoreactive T cell proliferation

Figure 1

Myelin-phagocytosing macrophages inhibit TCR-triggered lymphocyte proliferation in an antigen-independent manner. (a) Mean DiI-flourescence following treatment of macrophages with divergent concentrations of myelin (10 and 50 μg/ml) for several periods of time (1 hr; white bars, 2 hr; black bars or 3 hr; grey bars). The dotted line represents untreated macrophages. Data represent the mean of two independent experiments. (b, c) Lymphocyte proliferation and viability following a 4d coculture with irradiated thymocytes, purified MBP and different concentrations of macrophages. The relative proliferation and viability is defined as the percentage of proliferating or viable cells in experimental cultures divided by values in control lymphocyte cultures without macrophages (dotted line). Data represent the mean of three independent experiments. (d, e) Proliferation of MBP or OVA-reactive lymphocytes following a 4d coculture with irradiated thymocytes, macrophages or mye-macrophages (macrophage/lymphocyte ratio 1/10) and respectively purified MBP or OVA. Data represent the mean of six (d) and four (e) independent experiments. Dotted line represents the proliferation of control lymphocyte cultures without macrophages. Ma: Macrophages.

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