Figure 5
From: Myelin-phagocytosing macrophages modulate autoreactive T cell proliferation
MBP-immunized animals demonstrate an aggravated disease course following myelin treatment. (a) MBP-immunized animals were treated with isolated myelin at day -4, 0, 4 and 8 (or left untreated). Ten days post-immunization popliteal lymph node cultures were isolated and MBP (white bars) and MOG (black bars) reactivity was assessed. Non-stimulated cultures were used as control. Data represent the mean of two independent experiments. (b) Neurological score of control (N = 5) and myelin-treated (N = 5), immunized animals was assessed daily. Due to severity of paralysis, myelin-treated animals were sacrificed at d10. (c) LFB/cresyl violet staining of spinal cord (left) and brain tissue (right) of myelin-treated animals at day 10. Arrows depict demyelinated regions. One experiment is shown. Pop. LN: Popliteal lymph node.