OSM induces dose- and time-dependent IL-6 expression in human U343 glioma cells. OSM treatment leads to dose-dependent IL-6 release (A). U343 cells were stimulated with different concentrations of OSM as indicated. After 24 hours the conditioned medium was analyzed by IL-6-specific ELISA. The time-dependent profile of OSM treatment shows a significant increase in IL-6 mRNA expression and protein secretion (B). U343 cells were stimulated with 100 ng/ml OSM and harvested at time points indicated to extract total RNA or whole cell lysate. IL-6 mRNA expression was analyzed by qRT-PCR (dotted line, black triangles) and IL-6 protein release by ELISA (solid line, black squares). All data points, presented as mean ± SD, are from experiments carried out in quadruplicate. ns, not significant; *p < 0.05; ***p < 0.001.