Figure 3
From: Heteroarylketones inhibit astroglial interleukin-6 expression via a STAT3/NF-κB signaling pathway
Effect of PREP knock down on OSM-induced IL-6 expression in U343 cells. PREP knock down does not suppress OSM-induced IL-6 expression in U343 cells, whereas the compound HAK-2 reduces IL-6 levels under identical test conditions. U343 cells were transfected with PREP-specific siRNA and non-target control siRNA (NTC) or vehicle (control). After 48 h cells were transfected again for further 24 h followed by OSM-stimulation for 6 h. As positive control, only vehicle transfected cells were treated with compound HAK-2 (20 μM). Expression levels of PREP (A) and IL-6 mRNA (B) were measured by qRT-PCR, while conditioned media were used for quantification of IL-6 release by ELISA (C). ns, not significant; **p < 0.01; ***p < 0.001.