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Figure 2 | Journal of Neuroinflammation

Figure 2

From: HIV-1 Tat activates indoleamine 2,3 dioxygenase in murine organotypic hippocampal slice cultures in a p38 mitogen-activated protein kinase-dependent manner

Figure 2

Tat induces TNFα, IL-6, iNOS and SERT expression in OHSCs in a dose-dependent manner. Tat at a dose of 4, 40 and 400 ng/slice was added to the medium after 10 days in culture. Tissue and media were collected 6 h later. Average Ct values for 4, 40 and 400 ng/slice Tat were, respectively, for TNFα: 22.54 ± 0.68, 19.85 ± 0.43, 18.53 ± 0.43; IL-6: 21.34 ± 0.21, 19.88 ± 0.03, 19.29 ± 0.12; iNOS: 21.69 ± 0.14, 19.92 ± 0.14, 19.42 ± 0.26; SERT: 28.45 ± 0.04, 27.91 ± 0.02, 28.09 ± 0.15. Levels of TNFα and IL-6 (pg/ml) were measured in the supernatant by ELISA. Data represent the mean ± SEM (n = 3 in each group). Bars labeled with different letters (a, b, c or d) are significantly different from each other at p < 0.05.

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