Figure 6
From: Poly(I:C) promotes TNFα/TNFR1-dependent oligodendrocyte death in mixed glial cultures
Stimulation of mixed glia with TLR2 and TLR7 agonists increases TNFα and promotes preOL cytotoxicity. Rat mixed glial cultures were treated with vehicle (sterile PBS; 5 μl/ml), TLR1/2 agonist Pam3CSK4 (1.0 μg/ml), TLR3 agonist poly(I:C) (50 μg/ml), TLR4 agonist LPS (1.0 μg/ml) or TLR7/8 agonist R848 (5.0 μg/ml) for 48 h. PreOL viability was determined by counting O4+ cells. Scale bar, 50 μm. Results are combined means ± SEM from 4 independent experiments. *, p < 0.05, **, p < 0.01, ***, p < 0.001.