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Table 1 TNFα production in mixed glial cultures stimulated with TLR agonists

From: Poly(I:C) promotes TNFα/TNFR1-dependent oligodendrocyte death in mixed glial cultures

Stimulus TLR ligand TNFα (pg/ml) Significance
Control N/A 36.14 ± 3.112  
Pam3CSK4 TLR1/2 1865 ± 353.5 p < 0.01
poly(I:C) TLR3 1420 ± 73.39 p < 0.05
LPS TLR4 4645 ± 214.4* p < 0.001
R848 TLR7/8 1972 ± 361.1 p < 0.001
  1. Mixed glia were stimulated for 48 h with various TLR ligands and TNFα measured from culture supernatants by ELISA. Results are combined means ± SEM from 4 independent experiments. *LPS stimulation resulted in significantly higher TNFα production compared to all other TLR ligands (p 's < 0.001).