Skip to main content
Figure 7 | Journal of Neuroinflammation

Figure 7

From: TLR4 mutation reduces microglial activation, increases Aβ deposits and exacerbates cognitive deficits in a mouse model of Alzheimer's disease

Figure 7

TLR4M monocytes express less IL-1α, IL-1β, IL-6, CCL3 and CCL4 than TLR4W monocytes after fibrillar Aβ stimulation. CD11b+ splenocytes (monocytes) from 2-month-old TLR4W and TLR4 M Tg mice were collected by FACS. After treatment with fibrillar Aβ for 4 hours, mRNA levels of cytokines and chemokines were determined by real-time PCR. mRNA levels of cytokines and chemokines were normalized by subtracting cycle threshold (Ct) values obtained with GAPDH mRNA and are shown as 2 -ΔCt [ΔCt = Ct (cytokine or chemokine) - Ct (GAPDH)] (means ± SEM). *P < 0.005.

Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094