Figure 6

Inhibitory effects of ATL on NF-κB and AP-1 DNA-binding activities. BV-2 cells were pretreated with ATL for 30 min and stimulated with LPS for 1 h. Nuclear extracts were prepared and used to analyze NF-κB (A) and AP-1 (B) DNA-binding activity by EMSA, as described in Methods. Binding specificity was confirmed by unlabelled probe (100-fold in excess; lane 5) to compete with labelled oligonucleotide. The arrow indicates the NF-κB or AP-1 binding complex. Free-labelled probes are also indicated by an arrow. Results were confirmed by three independent experiments.