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Figure 2 | Journal of Neuroinflammation

Figure 2

From: Methyl salicylate 2-O-β- D -lactoside, a novel salicylic acid analogue, acts as an anti-inflammatory agent on microglia and astrocytes

Figure 2

The inhibitory effect of DL0309 on NO/iNOS induced by LPS in glial cells. Cells were pre-treated for 1 h with the indicated concentrations (μM) of DL0309, and then stimulated by LPS (0.5 μg/ml) for 24 h. Cell viability was determined by MTT assay (A). The level of nitrite in the culture medium was determined by the Griess reaction (B). iNOS protein levels in microglia (C) and astrocytes(D) were measured by western blotting. Data represent the means ± S.E.M. of three independent experiments. ###p < 0.001 vs. control; *p < 0.05, **p < 0.01 and ***p < 0.001 vs. LPS-treated cultures.

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