Figure 5

DL0309 decreases phosphorylated IKK levels in LPS-activated glial cells. Microglia (A) and astrocytes (B) were pre-treated for 1 h with the indicated concentrations of DL0309. LPS (0.5 μg/ml) was added and, 10 min later, proteins were isolated and the levels of phosphorylated IKKα/β, IKKα and IKKβ were measured by western blotting. Data represent the means ± S.E.M. of three independent experiments. ^#p < 0.05 vs. control; *p < 0.05 vs. LPS-treated cultures.