Figure 7

DL0309 blocks degradation of IκB by LPS-activated glial cells. Microglia (A) and astrocytes (B) were pre-treated for 1 h with the indicated concentrations of DL0309. LPS (0.5 μg/ml) was added and, 45 min later, proteins were isolated and levels of IκB-α were measured by western blotting. Data represent the means ± S.E.M. of three independent experiments. ^###p < 0.001 vs. control; *p < 0.05, **p < 0.01 and ***p < 0.001 vs. LPS-treated cultures.