Figure 1

Efficiency of DAI knockdown in primary murine microglia and astrocytes by small-interfering RNA. Microglia (Mg) and astrocytes (Ast) were untreated (Con) or transfected with a combination of three different siRNA duplexes targeting murine DAI using FuGENE HD transfection reagent. At 72 hours post-transfection, untreated and transfected cells were exposed to HSV-1 (MOI of 10) for 24 hours and DAI knockdown was confirmed in whole cell lysates by immunoblot analysis. Expression of a non-specific protein band observed following Coomassie blue staining is shown as a loading control (lc). For comparison purposes, DAI expression in murine small intestine tissue is shown (+).