Figure 2

Cultured primary murine astrocytes and microglia constitutively express the cytoplasmic viral sensor DAI and associated effector molecules, and its expression is elevated following viral challenge. Cultured microglia (Panels A and C) or astrocytes (Panels B and C) were untreated (0) or infected with HSV-1 (MOI of 0.1, 1 and 10) or MHV-68 (MOI of 1 and 10). At 24 hours post-infection expression of DAI (Panels A and B), RIP3 and STING (Panel C) were determined in whole cell lysates by immunoblot analysis. Expression of a non-specific protein band observed following Coomassie blue staining is shown as a loading control (lc) for each blot. For comparison purposes, DAI expression in murine small intestine tissue is shown (+). Representative results are shown for one of three separate experiments.