Figure 3
Ex vivo glial cells express DAI constitutively and/or inducibly following in vivo HSV-1 infection. Mice were sham-infected (0) or infected with HSV-1 (HSV: 1 × 105 PFU, i.n.). At 4 days post-infection protein isolates were prepared from whole brain tissue (Brain), or microglia (Mg) or astrocytes (Ast) acutely isolated by flow cytometry, and analyzed for the presence of DAI (Panels A and B), viral gG1 (Panel C), RIP3 and STING (Panel D) by immunoblot analysis. For comparison purposes, DAI expression in the murine neuron-like cell line CATH.a (Panel A: Cath) and murine small intestine tissue (Panels A and B: +), or viral gG1 expression in HSV-1 infected Vero cells (Panel C: +) is shown. Expression of a non-specific protein band observed following Coomassie blue staining is shown as a loading control (lc) for each blot. Immunoblots shown are representative of three separate experiments.