NCR1 expression in PBMC. (A) Representative panel indicating the gating strategy used to evaluate the expression of NCR1 on NK cells. First, lymphocytes were gated according to SSC vs FSC, then on NK cells defined as CD56+CD3- cells. NCR1 expression was evaluated on NK cells. The top plot shows a histogram for the isotype control and the bottom plot for the NCR1 stained cells. The expression of NCR1 was calculated as the ratio between the MFI of NK cell NCR1 expression and the isotype control for each sample. (B) Graph shows the MFI ratios calculated as described above for healthy controls, RRMS-untreated, RRMS-treated and PMS donors. A one-way ANOVA was conducted with Bonferroni's correction for multiple comparison tests and no significant difference was found (lower panel). There is a trend to lower expression in naive MS patients (MFI = 18.29 ± 4.734; p = 0.07) compared to controls (MFI = 28.10 ± 4.2761) which may have reach baseline levels after treatment (MFI = 21.70 ± 6.313).