Figure 4

NCR1 expression on astrocytes in WML. In the CNS, NCR1 was expressed on astrocytes and more so in WML from MS patients (A) than in controls (B). Immunoreactivity was quantified and a significant increase (p = 0.0003) in WML (4.945 ± 1.174) compared to controls (0.801 ± 0.27) was found when conducting a Mann Whitney test (C). NCR1 immunoreactivity was in general concentrated on reactive astrocytes near blood vessels and in some instances, NCR1 positive end-feet could be distinctly observed (D). In addition to stellate morphology, the phenotype of NCR1⁺ cells was confirmed using double immunofluorescence with a classical astrocyte marker, GFAP and with the mouse monoclonal to extracellular domain (E-G) and to full length NCR1 (H-J). Scale bar = 25 μm (A & B), Scale bar = 10 μm (D).