Figure 7
De novo expression of NCR1 mRNA in human U251 astrocytoma cells after stress. The human U251 astrocytoma cell line was used to look for de novo expression of NCR1 in astrocytes. Expression of NCR1 mRNA was detected when cells were cultures at a concentration of 2 × 106/well (A). No reverse transcription samples were negative, confirming that the bands were from de novo NCR1 mRNA production after stress and not from genomic DNA. Semi-quantitative analysis of NCR1 expression showed a 43% increase in NCR1 expression after 3 days of culture. Double immunofluorescence (GFAP-NCR1) confirmed presence of NCR1 protein on the human U251 astrocytoma cell line (C-E).