Microglial behavior in SCI is not correlated with neuronal death. Cross sections were obtained from the intact spinal cord (A, A’), epicenter (B, B’), and P1 regions (C, C’, C”) in the caudal direction at the indicated times after injury, and double-stained with anti-NeuN and anti-Iba-1 antibodies. NeuN and Iba-1 immunopositive regions were visualized with Alexa 488 (green)- and Alexa 555 (red)-conjugated secondary antibodies, respectively. (A’-C’) Higher magnification images of boxed regions in panels (A-C). (C”) Cross sections from the P1 regions were stained with TUNEL (green) and Iba-1 (red). Nuclei were counterstained with DAPI. Scale bars: 100 μm (A), 20 μm (A’) and 10 μm (C”). Data shown are representative of at least three independent experiments.