Telmisartan directly ameliorates the neuronal inflammatory response to IL-1β partly through the JNK/c-Jun and NADPH oxidase pathways

Table 1 List of PCR primers used in the study

Gene	Accession number	Forward primer (5′→3′)	Reverse primer (5′→3′)
hAT₁	S77410	ACCGCCCCTCAGATAATGTAAG	TGAAGTGCTGCAGAGGAATGTT
hCOX-2	NM_000963	GATTGCCCGACTCCCTTGG	AACTGATGCGTGAAGTGCTG
hGAPDH	NM_002046	CCCATCACCATCTTCCAGGAG	GTTGTCATGGATGACCTTGGCC
hIκB-α	NM_020529	CGGACTGCCCTTCACCTC	ACATCAGCCCCACACTTCAA
hIL-1R1	NM_000877	AGAGGAAAACAAACCCACAAGG	CTGGCCGGTGACATTACAGAT
hNOX-1	NM_013955	ATCACAACCTCACCTTCCAC	ATAGGCTGGAGAGAATGGA
hNOX-2	NM_000397	CCCTTTGGCACTGCCAGTGAAGAT	CAATCCCTGCTCCCACTAACATCA
hNOX-4	NM_016931	GGATCACAGAAGGTTCCAAGCAG	GCAGCCACATGCACGCCTGAGAA
hNOX-5	NM_024505	ATCAAGCGGCCCCCTTTTTTTCAC	CTCATTGTCACACTCCTCGACAGC
rAT_1A	NM_030985	AGCCTGCGTCTTGTTTTGAG	GCTGCCCTGGCTTCTGTC
rCOX-2	AF233596	CGGAGGAGAAGTGGGGTTTAGGAT	TGGGAGGCACTTGCGTTGATGG
rGAPDH	NM_017008	ATGACTCTACCCACGGCAAG	TGGAAGATGGTGATGGGTTT
rIL-1R1	NM_013123	TGAATGTGGCTGAAGAGCAC	CTTCCATCGTCTCATTCCGT

h, human; r, rat.
For AT₁ receptor mRNA expression, the products of PCR amplification were separated on 3 % agarose gel and visualized with ethidium bromide to verify the size of amplicon.

ISSN: 1742-2094