Neutrophil depletion does not prevent early mortality. (A) Neutrophil infiltration was characterized by flow cytometry based on CD45hi Ly6G+ expression (R4 region) in the CNS of controls (control (ctr); infected SCID mice without CD4+ T cell transfer), infected SCID recipients of WT (WT) or GKO CD4+ T cells (GKO) eight days p.i. (B) Mean expression (±SEM) of IFN-γ and CXCL1 mRNA analyzed in brains of naïve (n = 4), controls (n = 3), SCID recipients of WT (n = 4) or GKO (n = 4) CD4+ T cells at day eight p.i. Data are representative of two experiments. (C) Survival rates of SCID recipients of WT or GKO CD4+ T cells (with and without neutrophil depletion) observed daily following JHMV infection. Data represent the mean of sixteen mice per group combined from two separate experiments with n = 8 per group in each experiment. Efficiency of neutrophil (CD45hi Ly6G+, R4 region) depletion after antibody (Ab) treatment analyzed by flow cytometry in the CNS of SCID recipients of GKO CD4+ T cells at day eight p.i. Density plots are representative of eight animals per group.