Figure 2

Anti-IFN-γ induced CNS neutrophil infiltration but did not increase mortality in SCID recipients of WT CD4 ⁺ T cells. (A) Survival of infected SCID recipients of WT CD4⁺ T cells in the absence or presence of anti-IFN-γ mAb and recipients of GKO CD4⁺ T cells assessed daily until day ten p.i. Data are mean of at least six mice per group combined from two separate experiments (B) Anti-IFN-γ mAb efficiency assessed by flow cytometry by measuring MHC class II expression on microglia (CD45^lo F4/80⁺) in infected SCID recipients of WT (n = 6), WT + anti-IFN-γ mAb (n = 8) or GKO (n = 8) CD4⁺ T cells. Data are representative of two experiments. (C) Numbers of infiltrating neutrophils in the brain of infected SCID recipients of WT, WT + anti-IFN-γ mAb or GKO CD4⁺ T cells determined by flow cytometry at day eight p.i. Data represent the mean (±SD) from two independent experiments (n = 6, untreated group and n = 8, anti-IFN-γ treated group).