IL-17 expression in SCID recipients of GKO CD4
T cells. IL-6 and IL-1β (A), IL-17 (B), and IL-22 and IL-21 (C) mRNA expression analyzed by quantitative real-time PCR in brains of naïve (n = 4), infected control (n = 3), infected SCID recipients of WT (n = 4) or GKO (n = 8) CD4+ T cells at day eight p.i. IL-17 mRNA expression was measured in infected SCID recipients of WT CD4+ T cells + anti-IFN-γ mAb (n = 8). Data represent the mean (±SEM) from two separate experiments. (D) IL-17 (green) and CD3 (red) expression in the brains of SCID recipients of GKO CD4+ T cells. CD3+ (arrows) and IL-17-producing T cells (arrow heads) detected at day eight p.i. (E) IL-17-expressing CD4+ T cells from cervical lymph nodes of SCID recipients of GKO CD4+ T cells at day eight p.i. Dot plots are representative of four individuals. (F) Expression of IFN-γ and IL-17 by donor-derived CD4+ T cells prior to transfer analyzed by flow cytometry after in vitro stimulation. Intracellular cytokine expression measured using FITC-IFN-γ, PE-IL-17 and the corresponding isotype controls. Dot plots are representative of duplicates from two separate experiments. N.D= Not Detected.