Effects of chronic (8 months) hIVIG treatment on glial pathology in APdE9 mice. Immunostainings specific for astrocytes and microglial cells were performed on free-floating brain sections. Panel shows representative images from hippocampi of saline (n = 15) and hIVIG (n = 14-16) treated mice. (A) Astrocyte reactivity measured as GFAP immunoreactivity. (B) Microglial reactivity as measured as Iba-1 immunoreactivity. (C) Lysosomal marker CD68 immunoreativity. (D) Microglial activation as measured by CD45 immunoreactivity showed significant differences between the treatments. Scale bars represent 200 μm. Data are given as mean ± SEM. * P < 0.05, Student’s t-test. Scale bar represents 200 μm.