Figure 5
From: Dok2 mediates the CD200Fc attenuation of Aβ-induced changes in glia
Dok2 mediated the effects of CD200Fc. Efficiency of Dok2 siRNA knock-down was measured by western blot (A) and immunofluoresence (B). Incubation of microglia in the presence of Aβ significantly increased expression of CD40 mRNA (C) and release of IL-1β (D) and TNFα (E; **P < 0.01; ***P < 0.001; ANOVA; n = 6). Addition of CD200Fc to the incubation medium significantly attenuated these Aβ-induced changes (+ P < 0.05; ++ P < 0.01). Whereas non-target siRNA exerted no significant effect, Dok2 siRNA prevented the action of CD200Fc so that there was a significant difference in CD40 mRNA and in supernatant concentrations of IL-1β and TNFα prepared from cells treated with Aβ + CD200Fc compared with those treated with Aβ + CD200Fc + Dok2 siRNA (§ P < 0.05; ANOVA).